This training program includes hardware and software introduction Outline of the ICP-OES training.

Introduction to HPLC and basic principle of HPLC

Brief introduction to manual injection valves, isocratic pumps and UV detection

Make injection and run basic HPLC chromatogram.

Effects of changing eluent composition, flow rate, column and detector

Making up an accurate mobile phase composition

Make flow path connections

Basic precautions to avoid problems

HPLC solvent selection

Column: replacement, efficiency and capacity

Introduction to chromatographic interpretation

Introduction to sample preparation

Introduction to autosamplers

Safety: Working with small particle size materials, high pressures, UV radiation and electronic safety

Peak identification

Retention times and appropriate time windows for each peak