This course covers the basics of high performance liquid chromatography. All of the currently available HPLC components from the pumps to the detectors will be discussed. Various HPLC detectors & their uses, Binary, Isocratic gradients, Mobile phases, Polarity, Types of column, Retention Time, Linearity, Repeatability, Reproducibility. Troubleshooting to find out the problems are shown as they arise will also be shown – whether they are the fault of the system or the column. The modes of chromatography and their related columns and mobile phases used for various applications will be discussed. Preparation of standards, HPLC functioning & working.
Course Objective
The course provide thorough practical and theoretical in put to the trainee in HPLC. The difference in the column chemistries and their importance will be shown. Most applications shown will deal with the reversed phase mode, since it is the most used today.
Course Certificate
Spectro provide training certificates to the candidate.
Who should attend?
This course could be of interest to anyone who will be doing hands on HPLC. This might include researchers whose main field is not chemistry, chemists who have not done chromatography, or technicians who have new responsibilities in this area. All will learn from this basic HPLC course so they have a better understanding of the principles involved to get better results of their HPLC work.
Course Outline
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Chromatography theory |
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HPLC system components |
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Modes of chromatography |
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Column configurations |
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Care and maintenance of columns |
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UV/VIS detector functioning, its working, Calibrations & applications. |
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Fluorescence detector functioning, its working, Calibrations & applications. |
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Specific Applications |
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Maintenance & Troubleshooting |
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Questionnaire |
